Stabilization of particles and reduction of sample discordance in immunoassays using casein coating of particles
US6461874B1 · kind B1 · utility
4Cited by
24References
10Claims
0Family size
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Key dates
| Filing date | Jan 26, 2000 |
| Grant date | Oct 8, 2002 |
| Priority date | — |
| Expiry date | Jan 26, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2998
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used in immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and fewer discordant samples have been developed. Casein used at a concentration of 0.05-4.0 grams per gram of paramagnetic particle (optimally approximately 0.78-1.2 grams of casein per gram of magnetic particle) has been found to confer this benefit.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.