Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US6465175B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 3, 1998 |
| Grant date | Oct 15, 2002 |
| Priority date | — |
| Expiry date | Sep 3, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for reducing background signals encountered in nucleic acid hybridization assays and other assays that involve hybridization of a labeled oligomer to its complement. The method is premised on the significant reduction of signal generation that occurs when a quenchable dye-labeled oligomer forms a hybrid complex. In addition, a method is provided for enhancing the detectable signal emitted from an amplification multimer hybridized to an oligomer probe to which a quenchable dye has been conjugated through a linker such that the emission from the dye is not quenched upon hybrid complex formation. Novel oligonucleotide probes are also provided that comprise an oligomer to which has been directly or indirectly through a linker a quenchable dye.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.