Glycosylation using GalNac-T4 transferase

US6465220B1 · kind B1 · utility

Assignee

• GlycoZym ApS · DK

Inventors

• Frau Helle Hassan · Copenhagen, DK
• Henrik Clausen · Seattle, US
• Eric Paul Bennett · Avenue Madeleine, DK
• Detlef Eisenkrätzer · Lörrach, DE
• Jochen Gätgens · Jülich, DE

Key dates

• Filing date: Dec 21, 1998
• Grant date: Oct 15, 2002
• Expiry date: Dec 21, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The use of human polypeptide N-acetylgalactosaminytransferase T-4 (GalNAc-T4) to glycosylate substrates is disclosed. The action of GalNAc-T4 can complement the activities of GalNAc-T1, -T2 and -T3. The glycosylated substrates are useful in preparation of vaccines and anti-inflammatory agents. A method of producing the soluble form of the enzyme is also disclosed.