Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US6468742B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 12, 1999 |
| Grant date | Oct 22, 2002 |
| Priority date | — |
| Expiry date | Apr 12, 2019 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01L2924/0002
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphi…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.