Sequence-dependent gene sorting techniques

US6468749B1 · kind B1 · utility

Assignee

• Quark Biotech, Inc. · US

Inventors

• Levy Ulanovsky · Palo Alto, US
• Raja C. Mugasimangalam · Bengaluru, IN
• Paz Einat · Nes Ziona, IL
• Dina Zezin-Sonkin · Rehovot, IL
• Shlomit Gilad · Ganei Hadar, IL

Key dates

• Filing date: Mar 30, 2000
• Grant date: Oct 22, 2002
• Expiry date: Mar 30, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1096
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a method of sorting genes comprising: (1) preparing ds cDNA molecules from mRNA molecules (2) digesting the ds cDNA molecules (3) ligating to the digested cDNA molecules a set of dsDNA oligonucleotide adaptors; (4) amplifying the ligated cDNA molecules; and (5) sorting the amplified cDNA molecules into nonoverlapping groups. This invention also provides two additional methods of sorting genes. This invention further provides a method of making sub-libraries of ligation sets.