Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6469159B1 · kind B1 · utility
18Cited by
2References
15Claims
0Family size
Inventors
Key dates
| Filing date | Apr 26, 1999 |
| Grant date | Oct 22, 2002 |
| Priority date | — |
| Expiry date | Apr 26, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides rapid and highly effective methods for extracting nucleic acids suitable for PCR amplification from tissue samples and paraffin-embedded tissue samples. Extraction is accomplished within a few minutes using a composition comprising a buffer, at least one nonionic surfactant, and a protease enzyme. The sample is then heated at alkaline pH and after a centrifugation step, the DNA is the supernatant can be used directly in known amplification methods, such as PCR.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.