Diagnostic systems and method for determining the presence of a genotoxic and/or toxic compound in a sample

US6472152B1 · kind B1 · utility

Assignee

• Vlaamse Instelling voor Technologisch Onderzoek NV (VITO) · BE

Inventors

• Daniel Van Der Lelie · Shoreham, US
• Luc Agnes Louis Jean Bosco Regniers · Dessel, BE
• Safiyh Taghavi · Chapel Hill, US
• Philippe Corbisier · Mol, BE
• Luc Philippine Edouard Verschaeve · Brussels, BE

Key dates

• Filing date: Jan 27, 2000
• Grant date: Oct 29, 2002
• Expiry date: Jan 27, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6897
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention concerns a diagnostic system made of: a transformed microorganism capable of an increased reporter activity upon exposure to an environmental insult, said microorganism having a stress inducible promoter sequence being operatively linked to a reported encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed, and of a transformed microorganism having a constitutive and non stress inducible promoter sequence being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed.