Process for counting sperms and reagent therefor
US6472168B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 22, 2001 |
| Grant date | Oct 29, 2002 |
| Priority date | — |
| Expiry date | Feb 22, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5091
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for counting sperms (sperm cells) comprises the steps of: (1) mixing a specimen containing sperms with an aqueous solution containing a cationic surfactant in an amount effective in removing contaminants contained in the specimen to give a surfactant-treated specimen, (2) staining the sperms contained in the surfactant-treated specimen with a staining liquid containing a dye for staining nucleic acid to give a measuring specimen, (3) introducing the measuring specimen into a flow cell in a flow cytometer and irradiating the stained sperms in the measuring specimen with excitation light, (4) detecting a scattered light signal and a fluorescent light signal emitted from the sperms irradiated with the excitation light, (5) preparing a two-dimensional distribution involving two axes of a scattered light intensity and a fluorescent light intensity based on the scattered light signal and the fluorescent light signal, and (6) specifying a region of a sperm mass on the two-dimensional distribution and counting the number of sperm in the region of the sperm mass.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.