Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle

US6475729B1 · kind B1 · utility

Assignee

• Johnson & Johnson Clinical Diagnostics, Inc. · US

Inventors

• John Bruce Findley · Rochester, US
• John W. Backus · Williamson, US
• William Harold Donish · Rochester, US
• John W. Sutherland · Rochester, US

Key dates

• Filing date: Oct 29, 1999
• Grant date: Nov 5, 2002
• Expiry date: Oct 29, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure. This procedure includes a series of steps which have critically defined temperature and time parameters. Each polymerase chain reaction cycle requires generally less than about two minutes, and in most cases less than 90 seconds. At least 5 units/100 &mgr;l of solution of thermostable DNA polymerase are used, and other preferred levels of primer concentrations facilitate the quick cycling in the amplification. In preferred embodiments, only two temperatures are used in the amplification.