Scintillation proximity assay for type II fatty acid biosynthetic enzymes
US6475751B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 5, 2001 |
| Grant date | Nov 5, 2002 |
| Priority date | — |
| Expiry date | Apr 15, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides scintillation proximity assays (SPAs) for measuring the activity of enzymes involved in type II fatty acid biosynthesis. Enzymes which can be assayed in this manner include KASI, KASII, KAS III and MAT. The present invention also provides methods of assaying for ketoacyl synthase activity, malonyl transferase activity, and acyl transferase activity in enzymes using the SPA format. Because the SPA format allows the direct detection of a radiolabeled product, these assay methods are appropriate for use in high throughput screening techniques. The present invention also provides methods for assessing a compound's ability to modulate the activity of a type II fatty acid biosynthetic enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.