Recombinant collagenase type I from clostridium histolyticum and its use for isolating cells and groups of cells

US6475764B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Helmut Burtscher · Habach, DE
• Dorothee Ambrosius · Munich, DE
• Friederike Hesse · Munich, DE

Key dates

• Filing date: Sep 9, 1999
• Grant date: Nov 5, 2002
• Expiry date: Sep 9, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2509/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A polypeptide with the properties of collagenase class I from Clostridium histolyticum (CHC I) and the amino acid sequence SEQ ID NO:2 which is optionally N-terminally extended by one or several amino acids of the sequence SEQ ID NO:3 is advantageously suitable for the isolation of cells from mammalian tissue and human tissue.