Patent · US Expired

Amphotropic retrovirus packaging cell line, process for its production and use thereof

US6475786B1 · kind B1 · utility

1Cited by

2References

4Claims

0Family size

Assignee

Roche Diagnostics GMBH · DE

Inventors

Claudio Bordignon · Milano, IT
Bernhard Goller · Penzberg, DE
Ruediger Rueger · Seeshaupt, DE
Georg Tiefenthaler · Sindelsdorf, DE

Key dates

Filing date	Jun 11, 2001
Grant date	Nov 5, 2002
Priority date	—
Expiry date	Jun 11, 2021

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2740/13052
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A titer of retroviral vectors of at least about 107 colony-forming units/ml cell culture supernatants is obtained using an amphotropic retroviral packaging cell line which contains functional gag, pol and env genes integrated into the genome in which the expression of the genes gag and pol is regulated independently of the expression of the env genes, wherein the genome of the said cell line contains one or two functionally active env genes.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.