Biosensor device and method
US6478939B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | May 24, 2000 |
| Grant date | Nov 12, 2002 |
| Priority date | — |
| Expiry date | May 24, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/908
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an &agr;-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.