Culturing different cell populations on a decellularized natural biostructure for organ reconstruction
US6479064B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 29, 1999 |
| Grant date | Nov 12, 2002 |
| Priority date | — |
| Expiry date | Dec 29, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
artificial organs are reconstructed using a three-dimensional scaffold produced by decellularizing biostructures from a donor organ. The three-dimensional scaffold is perfused with isolated endothelial cells that develop to produce an endothelial tissue layer with a primitive vascular system that sustains the growth and development of a second cultured cell population. When grown in the three-dimensional scaffold containing the endothelial tissue layer, with the primitive vascular system, the cells of the second cell population proliferate, mature and differentiate into neomorphic organ structures that are analogous to their in vivo counterparts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.