Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
US6479243B1 · kind B1 · utility
11Cited by
2References
23Claims
0Family size
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Key dates
| Filing date | Mar 5, 2001 |
| Grant date | Nov 12, 2002 |
| Priority date | — |
| Expiry date | Mar 5, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed is an in vitro process for synthesizing DNA encoding a family of antigen-combining proteins. This process involves obtaining DNA containing genes encoding antigen-combining proteins and then combining these genes with sequence specific primers. These primers can be oligonucleotides homologous to conserved regions of the genes. The genes and primers are then subjected to sequence specific gene amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.