Method of determining cholesterol content of high-density lipoproteins
US6479249B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 6, 1998 |
| Grant date | Nov 12, 2002 |
| Priority date | — |
| Expiry date | Aug 10, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/824
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for quantifying cholesterol in HDL, which does not require complex fragmentation and separation operations, and by which the HDL cholesterol in test samples containing HDL and other lipoproteins such as low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicron (CM) may be quantified selectively, simply and accurately. The method for quantifying cholesterol in high density lipoprotein comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein in a test sample, and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of the first step and enzymatically quantifying cholesterol in high density lipoprotein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.