Methods and kits for isolating primer extension products using modular oligonucleotides

US6482592B1 · kind B1 · utility

Assignee

• Dynal AS · NO

Inventors

• Joakim Lundeberg · Lidingö, SE
• Mathias Uhlen · Uppsala, SE

Key dates

• Filing date: Sep 15, 1998
• Grant date: Nov 19, 2002
• Expiry date: Sep 15, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6832
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a method of improving the binding of a series of consecutive nucleotide bases to a complementary target nucleic acid molecule in a sample, wherein said method comprises at least the step or steps of binding a complementary modular oligonucleotide of at least two parts (modules) including said nucleotide bases to adjacent stretches of said target nucleic acid molecule in said sample, especially methods of detection/isolation, particularly in the isolation of primer extension products and methods in which the modular oligonucleotide is a primer, modular oligonucleotides themselves and their use in methods of the invention.