Method for measuring antigen concentration
US6482596B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 18, 1999 |
| Grant date | Nov 19, 2002 |
| Priority date | — |
| Expiry date | May 18, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6857
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptide onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.