Amylolytic enzyme variants
US6482622B1 · kind B1 · utility
7Cited by
1References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 24, 2000 |
| Grant date | Nov 19, 2002 |
| Priority date | — |
| Expiry date | Aug 24, 2020 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA21D8/042
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.