Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof

US6485912B1 · kind B1 · utility

Assignee

• The Rockefeller University · US

Inventors

• Nathaniel Heintz · Pelham Manor, US
• Weining Jiang · New York, US
• Xiangdong Yang · Beijing, CN

Key dates

• Filing date: Aug 24, 2000
• Grant date: Nov 26, 2002
• Expiry date: Aug 24, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/42
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of efficiently sequencing multiple exons from complex genomic DNAs is disclosed. The methodology includes the use of bacterial and bacteriophage-derived artificial chromosomes (BBPACS) in novel gene trapping protocols. Targeted gene trapping by homologous recombination, and random gene trapping with the use of a transposon system are exemplified. Included in the invention are methods of preparing a gene map from BBPAC contigs, the resulting gene maps, methods of constructing a cDNA library from BBPAC contigs, and the resulting cDNA libraries.