Analytical element for species-specific detection of nucleic acids

US6485915B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Volker Ernst Willi Keller · Speyer, DE
• Andreas Rauscher · Ludwigshafen, DE
• Joachim Steinbiss · Lorsch, DE
• Reiner Schlipfenbacher · Lampertheim, DE
• Jürgen Klepp · Karlsruhe, DE

Key dates

• Filing date: Nov 17, 2000
• Grant date: Nov 26, 2002
• Expiry date: Nov 17, 2020

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention concerns a method for the detection of nucleic acids on an analytical element which contains a sample application zone and a detection zone, the analytical element enabling liquid transport from the sample application zone to the detection zone, wherein a sample is applied to the sample application zone of the analytical element and the nucleic acids contained in the sample are denatured and can be detected qualitatively in the detection zone by hybridization with a detection probe and also quantitatively, preferably by means of marker groups. In addition new analytical elements and reagent kits for the detection of nucleic acids are provided.