Method for altering antibody light chain interactions
US6485943B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 1999 |
| Grant date | Nov 26, 2002 |
| Priority date | — |
| Expiry date | Mar 22, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.