Production of lactate using crabtree negative organisms in varying culture conditions

US6485947B1 · kind B1 · utility

Assignee

• Cargill Dow Polymers LLC · US

Inventors

• Vineet Rajgarhia · Dublin, US
• Vassily Hatzimanikatis · Minneapolis, US
• Stacey Olson · Minneapolis, US
• Ting Liu Carlson · Dayton, US
• John N. Starr · Chaska, US
• Jeffrey John Kolstad · Wayzata, US
• Aharon Eyal · Jerusalem, IL

Key dates

• Filing date: May 19, 2000
• Grant date: Nov 26, 2002
• Expiry date: May 19, 2020

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Crabtree negative organisms such as Kluyveromyces, Pichia, Hansenula and Candida, are used to make selected organic products such as lactic acid. The organisms are cultured in a first culture medium that includes glucose, under conditions that promote cellular respiration. The organisms are then cultured under a second set of conditions that promote production of the selected organic product. The organisms preferably contain an exogenous lactate dehydrogenase gene.