Method of detecting sequence variations in macromolecules by thermal gradiant
US6486309B1 · kind B1 · utility
5Cited by
2References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 1, 2001 |
| Grant date | Nov 26, 2002 |
| Priority date | — |
| Expiry date | Aug 1, 2021 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D15/366
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention describes a method for separating macromolecules, particularly heteroduplex from homoduplex DNA molecules. The macromolecules undergo a temperature sensitive structural denaturation which, when it occurs, results in a difference in the mobilities of the denatured components on a solid support. A polar to non-polar solvent gradient or modified solid support particular to each sample is not required, since the difference in mobility is achieved on conventional solid supports using a single mobile phase composition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.