Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US6489114B2 · kind B2 · utility
178Cited by
32References
25Claims
0Family size
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Key dates
| Filing date | Dec 18, 2000 |
| Grant date | Dec 3, 2002 |
| Priority date | — |
| Expiry date | Apr 9, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at least one of the 3′ end and the 5′ end of each of a plurality of the RNA fragments, the terminal phosphate having been released during the fragmentation, and binding a plurality of labeling agents to the first ligand on each of a plurality of the fragments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.