Retroviral packaging cassettes amplified in the cytoplasm by autocatalytic Togavirus vectors

US6489167B1 · kind B1 · utility

Assignee

• The Government of the United States as represented by the Secretary of the Department of Human Services · US

Inventors

• Richard A. Morgan · Vienna, US
• Jarmo Wahlfors · Columbia, US
• Kleanthis Xanthopoulos · Village of La Jolla, US

Key dates

• Filing date: Jul 12, 1999
• Grant date: Dec 3, 2002
• Expiry date: Jul 12, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/13052
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a Togavirus-amplified retrovirus vector and a novel method for packaging a retrovirus cassette that contains a heterologous nucleic acid, which is amplified in a packaging cell cytoplasm by a Togavirus vector. The retroviral cassette is packaged into infectious retrovirus particles by retroviral packaging cells. These retroviral particles carrying the retroviral packaging cassette are then used to infect host cells.