Method of determining peptide sequences by mass spectrometry
US6489608B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 6, 2000 |
| Grant date | Dec 3, 2002 |
| Priority date | — |
| Expiry date | Apr 6, 2020 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining the sequence of amino acids that constitute peptides, polypeptides or proteins by mass spectrometry and especially by tandem mass spectrometry is disclosed without the use of any additional data concerning the nature of the peptide and without any limit to the number of possible sequences considered. The method can be implemented on a personal computer typically used for data acquisition on the tandem mass spectrometer even in the case of peptides comprising 10 or more amino acids. The method does not rely on exhaustive comparison of the spectra predicted from every possible amino acid sequence with any molecular weight constraint, but instead uses mathematical techniques to simulate the effect of such a complete search without actually carrying it out.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.