Method for culturing a basidiomycetous fungus in a liquid culture medium
US6490824B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 12, 2001 |
| Grant date | Dec 10, 2002 |
| Priority date | — |
| Expiry date | Oct 12, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M41/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth-supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30% by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.