Assay for identifying inhibitors of the interaction between proteins p53 and dm2
US6492116B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 26, 2000 |
| Grant date | Dec 10, 2002 |
| Priority date | — |
| Expiry date | Jun 26, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5011
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention concerns a new assay which allows the identification of compounds which inhibit the formation of complexes between a product of the double minute 2 gene (“dm2”) and p53 but not between p53 and DNA. Both the complex formation of labeled DNA, C-terminally truncated p53 and dm2 and disruption of dm2 from the labeled DNA-p53 complex by an inhibitor of the p53-dm2 interaction can be detected by a gel shift assay procedure. This assay permits the selection of compounds which, besides their inhibitory property, do not alter p53 specific DNA binding and do not disturb p53 conformation required for DNA binding or formation of active tetramer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.