Methods of immobilizing ligands on solid supports
US6492118B1 · kind B1 · utility
160Cited by
22References
9Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 28, 2000 |
| Grant date | Dec 10, 2002 |
| Priority date | — |
| Expiry date | Aug 28, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54353
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for immobilizing a ligand, e.g., a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.