Method for producing polyhydroxyalkanoates in recombinant organisms
US6492134B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 15, 2000 |
| Grant date | Dec 10, 2002 |
| Priority date | — |
| Expiry date | Mar 15, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1029
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present biotechnological approach for the production of polyhydroxyalkanotes (PHAs) uses microbial systems. The high production costs makes them substantially more expensive than synthetic plastics. Engineering a novel pathway in eucaryotic cell systems is a beneficial alternative to the production of PHAs in bacteria. This pathway will initially produce free (C8) fatty acids from the fatty acid synthetic pathway through the action of thioesterase, that will then add a CoA moiety to the free fatty acid through the action of an acyl-CoA synthetase, that will produce 3-ketoacyl-CoAs from the acyl-CoA through the action of a thiolase, that will produce R-(−)-OH-acyl-CoAs from the 3-keto acid CoAs through the action of a dehydrogenase isoform from yeasts. These R-(−)-3-OH-acyl-CoAs will finally be used as substrate for the PHA synthase reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.