Patent · US Expired

Methods of initiating embryogenic cultures in plants

US6492174B1 · kind B1 · utility

19Cited by
12References
43Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 11, 2000
Grant dateDec 10, 2002
Priority date
Expiry dateOct 11, 2020

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N5/04
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention provides methods for initiating embryogenic cultures of plants. The methods include the use of novel media compositions and elevated atmospheric pressure treatments to improve the frequency of embryogenic culture initiation. The methods are well suited for initiating embryogenic cultures in recalcitrant conifer varieties. The method is also well suited for producing somatic embryos that can be further cultured to produce large numbers of plants. Further, the invention provides novel methods that may be used to enhance somatic embryogenesis in a broad range of species.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.