Oligopeptide derivatives for the electrochemical measurement of protease activity
US6495336B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2001 |
| Grant date | Dec 17, 2002 |
| Priority date | — |
| Expiry date | Nov 21, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96463
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to oligopeptide derivatives whose C-terminal amino acid is arginine which is linked to the remainder of an electroactive aniline or aminoquinoline derivative by an amide bond. The oligopeptide derivatives are split by enzymes of the class of peptide hydrolases, especially proteinases and their inhibitors, of the coagulation system, the fibrinolytic system and the complement system. These oligopeptide derivatives serve as substrates for quantitatively determining such enzymes, especially thrombin, in complex sample liquids, especially capillary blood. This determination is carried out by measuring the increase in the water-soluble amperogenic aniline or aminoquinoline compound. The oligopeptide derivatives and their salts can be produced according to usual methods in peptide chemistry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.