Mitomycin biosynthetic gene cluster

US6495348B1 · kind B1 · utility

Assignee

• Regents of the University of Minnesota · US

Inventors

• David H. Sherman · Ann Arbor, US
• Yingqing Mao · Windom, US
• Mustafa Varoglu · Windom, US
• Min He · Dongguan, CN
• Paul Sheldon · Brockville, CA

Key dates

• Filing date: Mar 12, 1999
• Grant date: Dec 17, 2002
• Expiry date: Mar 12, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a biosynthetic gene cluster for mitomycin, for example, a mitomycin biosynthetic cluster from organisms such as Streptomyces, for instance, S. lavendulae, as well as methods of using gene(s) within the cluster to alter antibiotic biosynthesis and to prepare a polyketide synthase.