Methods for producing transgenic pigs by microinjecting a blastomere
US6498285B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 5, 1998 |
| Grant date | Dec 24, 2002 |
| Priority date | — |
| Expiry date | Aug 5, 2018 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2217/05
- WIPO fieldOther special machines
- WIPO sectorMechanical engineering
Abstract
A transgenic large mammal is produced by a method including the steps of obtaining one or more early embryos, selectively preparing an embryo having at least three cells, and preferably at a stage in development corresponding in time to the onset of transcription of the embryo's paternal genome, and introducing isolated nucleic acid molecules into a blastomere of the selected embryo. The introduction of isolated nucleic acid molecules into such embryos results in the generation of transgenic large mammals at a significantly increased frequency as compared to introducing isolated nucleic acid molecules into zygotes or into the blastomeres of embryos at the one or two cell stage of development.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.