Metabolic controlled fermentation procedure for the manufacture of lovastatin hydroxy acid
US6500651B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 5, 2000 |
| Grant date | Dec 31, 2002 |
| Priority date | — |
| Expiry date | Apr 5, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P17/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing mevinolin by a microorganism in a fermentation process having a seed culture stage and a main fermentation stage, includinga) cultivating a microorganism biomass in the seed culture stage to produce an inoculum;b) transferring the inoculum into a fermentation medium in the main fermentation stage; and,c) maintaining steady stage conditions in the main fermentation stage, thereby producing a fermentation broth containing mevinolin. Preferably, the steady state conditions are maintained in the main fermentation stage by one or more of feeding of organic carbon sources; controlling glucose and/or total reducing sugar content; feeding of organic nitrogen sources; controlling pH; controlling foam level; controlling the mass of the fermentation broth by withdrawals and feedings; and, controlling the dissolved oxygen level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.