Method for the diagnosis of brain/neurological disease using monoclonal antibodies specific for PHF-tau, hybridomas secreting them, and antigen recognition by these antibodies and their applications

US6500674B1 · kind B1 · utility

Assignee

• Innogenetics N.V. · BE

Inventors

• Marc Vandermeeren · Geel, BE
• Eugeen Vanmechelen · Zevergem, BE
• Andre Van De Voorde · Lokeren, BE

Key dates

• Filing date: Nov 2, 1999
• Grant date: Dec 31, 2002
• Expiry date: Nov 2, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/4711
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for the diagnosis of brain/neurological disease involving abnormally phosphorylated tau protein using at least one antibody chosen from the group consisting of monoclonal antibody AT180 secreted by the hybridoma deposited at ECACC on Dec. 22, 1992 under No. 92122204, and monoclonal antibody AT270 secreted by the hybridoma deposited at ECACC on Jul. 7, 1993 under 93070774, each of which forms an immunological complex with a phosphorylated epitope of an antigen belonging to abnormally phosphorylated tau protein (PHF-tau) residing in the region spanning positions 143-254 with the following amino acid sequence:(SEQ ID NO 1)     143                         150NH2- Lys Gly Ala Asp Gly Lys Thr Lys Ile Ala Thr                         160Pro Arg Gly Ala Ala Pro Pro Gly Gln Lys Gly Gln            &#8…