Fibrin microbeads prepared from fibrinogen, thrombin and factor XIII for binding cells

US6503731B2 · kind B2 · utility

Assignee

• Hadasit Medical Research and Development Ltd. · IL

Inventors

• Gerard Marx · New York, US
• Raphael Gorodetsky · Jerusalem, IL

Key dates

• Filing date: Sep 15, 1998
• Grant date: Jan 7, 2003
• Expiry date: Sep 15, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/56
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Fibrin microbeads are prepared containing extensively cross-linked fibrin (ogen) without using glutaraldehyde. The fibrin microbeads are preferably prepared by containing an aqueous solution containing fibrinogen, thrombin and Factor XIII with an oil heated to about 50-80° C. to form an emulsion and mixing the emulsion at 50-80° C. until fibrin microbeads containing extensively cross-linked fibrin (ogen) are obtained. The fibrin microbeads may have a diameter of about 50-200 microns and can contain a bioactive agent. The fibrin microbeads are used for binding cells such as when culturing or separating one cell type from another, or when transplanting cells or engineering tissue.