Analytical element and method for the determination of an analyte in a liquid

US6506575B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Wolfgang-Reinhold Knappe · Ludwigshafen, DE
• Rudolf Pachl · Ellerstadt, DE
• Otto Gaa · Worms, DE

Key dates

• Filing date: Sep 22, 2000
• Grant date: Jan 14, 2003
• Expiry date: Sep 22, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/525
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns an analytical element for determining the amount of an analyte in a liquid containing a sample application zone (1) and a detection zone (2) which are in liquid-transferring contact, the latter containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and containing a liquid-permeable interference-reducing layer (3) in direct contact with the detection zone (2) arranged such that liquid does not reach the detection zone until it has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance, and a method for the determination of an analyte in a liquid by means…