Method of DNA transfer
US6506596B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Apr 26, 2001 |
| Grant date | Jan 14, 2003 |
| Priority date | — |
| Expiry date | Apr 26, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16034
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method of of transferring genomic DNA from apoptotic bodies to engulfing cells, wherein DNA is transferred from a donor cell to a recipient cell. More specifically the method includes providing somatic donor cells comprising desired DNA; generating apoptotic bodies of said donor cells; incubation of the apoptotic bodies with engulfing recipient cells under biological conditions allowing uptake of DNA from the apoptotic bodies by said recipient cells; and optionally selecting recipient cells which have integrated DNA from the apoptotic bodies. The present method is useful in various pharmaceutical applications, such as in vaccine preparations and gene identification procedures. Further, the present invention also relates to a method of preventing and/or treating a clinical condition in a patient, which comprises isolating cells comprising genomic DNA; generating apoptotic bodies of said isolated cells; incubation of said apoptotic bodies with engulfing recipient cells under biological conditions allowing uptake of DNA from the apoptotic bodies, optionally, selecting cells containing DNA originating from the isolated cells; and administering cells i…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.