Use of high frequency ultrasound imaging to detect and monitor the process of apoptosis in living tissues, ex-vivo tissues and cell-culture
US6511430B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 8, 2001 |
| Grant date | Jan 28, 2003 |
| Priority date | — |
| Expiry date | May 8, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2291/02854
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A non-invasive method of monitoring apoptosis in cell culture, ex-vivo tissues and in-vivo tissues using high frequency ultrasound imaging is provided. The method includes the steps of: 1) imaging a selected site of the cell culture or tissues using high frequency (above 20 MHz) ultrasound imaging (before image); 2) exposing the selected site to an apoptosis-inducing stress; 3) imaging the selected site or a portion thereof, using ultrasound imaging at subsequent timed intervals (after image(s)); 4) measuring the signal amplitude of a region of interest of the selected site in the before and after images; 5) comparing the signal amplitude measurements for the regions of interest in the before and after images and determining whether the after image regions exhibit an increase in amplitude as compared to the before image regions which is an indication that apoptosis has begun; and 6) measuring the change in the frequency spectrum of the radiofrequency ultrasound backscatter signal in the region of interest in the before and after images and confirming that apoptosis has begun when the slope of the frequency spectrum has increased.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.