Method for the detection of an analyte by means of a nucleic acid reporter

US6511809B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Luis Baez · Mountain View, US
• Richard C. Ebersole · Wilmington, US
• Edwin R. Hendrickson · Hockessin, US
• Neel Neelkantan · Newark, US
• Michael P. Perry · Downingtown, US

Key dates

• Filing date: May 16, 2001
• Grant date: Jan 28, 2003
• Expiry date: May 16, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6804
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process is disclosed for the detection of an analyte utilizing a nucleic acid label as a reporter. The analyte is detected by the binding of at least two reporter conjugates, each conjugate comprising a member of a binding pair and a nucleic acid label. The binding of the reporter conjugates to the analyte facilitates the juxtaposition of the nucleic acid labels, forming a single nucleic acid amplicon. The amplicon may then be detected directly, or may be used as a template of the generation of amplification products. Detection of the analyte by this process significantly reduces assay background caused by non-specific reporter conjugate binding.