Method for quantitating competitive binding of molecules to proteins utilizing fluorescence polarization
US6511815B1 · kind B1 · utility
10Cited by
1References
9Claims
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Key dates
| Filing date | Dec 20, 1999 |
| Grant date | Jan 28, 2003 |
| Priority date | — |
| Expiry date | Dec 20, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The system comprises mixing a fluorescence-emitting compound that binds to the steroid hormone receptors in a solution containing the steroid hormone receptors. Then, measuring the fluorescence polarization of the solution. Subsequently, incubating the solution with at least one molecule that may compete with the compound for interaction with the steroid hormone receptors. Measuring the fluorescence polarization of the solution again. Finally, comparing the fluorescence polarization measurements to quantify any competitive interaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.