Methods for enhancing plant transformation frequencies

US6512165B1 · kind B1 · utility

Assignee

• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Margit C. Ross · West Des Moines, US
• Laura A. Church · Des Moines, US
• Patrea M. Hill · Des Moines, US
• William J. Gordon-Kamm · Urbandale, US
• Keith Lowe · Johnston, US
• George J. Hoerster · Des Moines, US
• Dennis L. Bidney · Urbandale, US

Key dates

• Filing date: Jul 10, 2000
• Grant date: Jan 28, 2003
• Expiry date: Jul 10, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8207
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides improved plant transformation methods. In particular the method provides increased transformation freequency, especially in recalcitrant plants. The method comprises stably transforming a target cell with at least one polynucleotide of interest. The target cell has been previously transformed to stimulate growth of the cell and has gone through at least one cell division.