Nucleic acid detection using degradation of a tagged sequence
US6514700B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 21, 2000 |
| Grant date | Feb 4, 2003 |
| Priority date | — |
| Expiry date | Jun 21, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/08
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods and compositions are provided for detecting target molecules, e.g. DNA sequences, particularly single nucleotide polymorphisms, using a pair of nucleotide sequences, a primer and a snp detection sequence, where the snp detection sequence binds downstream from the primer to the target DNA in the direction of primer extension, or ligands and receptors. The methods employ e-tags comprising a mobility-identifying region joined to a detectable label and a target-binding region. The result of the binding of the target-binding region to the target is to have a bond cleaved in the starting material with the production of a detectable product with a different mobility from the starting material, where the different e-tags can be separated and detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.