Patent · US Expired

Human myoblast cell lines and their uses

US6514756B1 · kind B1 · utility

2Cited by
0References
6Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 27, 2000
Grant dateFeb 4, 2003
Priority date
Expiry dateApr 27, 2020

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2510/04
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Described is a human muscular cell line which is able to proliferate and generated from primary human muscular cells by a process comprising the step of:a) pre-treating a culture of said primary human muscular cells or a suspension thereof with at least one glucocorticoid,b) optional step comprising obtaining a suspension of said pre-treated culture of step a),c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells andd) culturing the transferred cells of step c).Furthermore, human muscular cells derived from said cell lines are provided as well as pharmaceutical and diagnostic compositions comprising the same.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.