Method for enhancing the hybridization efficiency of target nucleic acids using a self-addressable, self-assembling microelectronic device
US6518022B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 1999 |
| Grant date | Feb 11, 2003 |
| Priority date | — |
| Expiry date | Nov 22, 2019 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01L2924/10253
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.