Enzyme combinations for destroying proliferative cells
US6518062B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 10, 1998 |
| Grant date | Feb 11, 2003 |
| Priority date | — |
| Expiry date | Sep 10, 2018 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K48/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Enzyme combinations useful for destroying cells, particularly proliferative cells, are disclosed. In particular, a combination of the following enzymes has been found to enhance the toxicity of nucleoside analogues to proliferating cells: an enzyme that phosphorylates the non-toxic nucleoside analogue to generate a monophosphate analogue, an enzyme that phosphorylates the monophosphate analogue to generate a diphosphate analogue and an enzyme that phosphorylates the diphosphate analogue to generate a toxic triphosphate analogue. Vectors enabling the intracellular expression and transfer of the enzyme combinations, as well as their therapeutic use, particularly in anti-cancer gene therapy, are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.