Gene construct and its use

US6521449B1 · kind B1 · utility

Assignee

• GSF-Forshungszentrum für Umwelt und Gesundheit, GmbH · DE

Inventors

• Axel Polack · Hersbruck, DE
• Konstanze Hörtnagel · Hersbruck, DE
• Jurgen WOLF · Recklinghausen, DE
• Susanne Mücke · Recklinghausen, DE

Key dates

• Filing date: Nov 4, 1999
• Grant date: Feb 18, 2003
• Expiry date: Nov 4, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/46
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Compositions of matter and methods for expressing a polypeptide are provided. In one aspect, a gene construct is provided that comprises an enhancer comprising particular elements or combinations of elements from the immunoglobulin &kgr; locus, the immunoglobulin heavy chain &mgr; locus, and the immunoglobulin &lgr; locus, and a promoter and polyadenylation site linked to a nucleotide sequence encoding a polypeptide from a select group. Cells comprising the gene construct, processes for producing such cells, and processes for producing the polypeptide encoded by the gene construct from such cells are also provided.