High efficiency methods for combined immunocytochemistry and in-situ hybridization
US6524798B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 2000 |
| Grant date | Feb 25, 2003 |
| Priority date | — |
| Expiry date | Nov 26, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a high efficiency method for combined immunocytochemistry and in situ hybridization. In one aspect, the method is used to simultaneously determining a cell phenotype and genotype by contacting a cell with an antigen-specific antibody bound to a ligand, contacting the cell with polynucleotide probe to form a complex of the probe and a nucleic acid in the cell, contacting the cell with a detectably labeled anti-ligand, and detecting the polynucleotide-probe complex and the anti-ligand-ligand complex. The presence of the anti-ligand is correlated with the presence of the antigen and the presence of the probe-nucleic acid complex is correlated with the presence of the nucleic acid in the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.